buy clomid and nolvadex online The RNA was reverse transcribed and real time PCR products were generated in 35 cycles from 15 ng cDNA in an ABI Prism 7700 apparatus Applied Biosystems, Foster City, CA in a mixture containing SYBR green Applied Biosystems and 330 nmol L primers for differentially expressed genes ie, CASP2, DLX2, EZH1, CHD6, MST4, RABEP, SIAH2, and TNC